SERVICE

Custom Protein Service

分析服务

分析服务
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Protein Purification & Characterization

提炼蛋白质与特性描述

本公司对各类细菌与酵母细胞中发现的医药品重组蛋白质提炼工艺拥有丰富的经验。
不仅提供蛋白质提炼服务,还提供医药品研发过程中必需的蛋白质特性分析服务。

蛋白质提炼方法

  • Column chromatography
    – Gel filtration chromatography
    – Ion exchange chromatography
    – Hydrophobicity chromatography
    – Affinity chromatography
  • High-pressure Liquid Chromatography (HPLC)

蛋白质特性分析服务项目

  • SDS-PAGE
  • Isoelectric focusing
  • Western Blot analysis
  • ELISA
  • Intact mass determination (MALDI-TOF)
  • Peptide mapping
  • Post-translation modification analysis
  • Disulfide bond localization
  • Impurity identification
  • N-glycan Analysis

欲知详情请致电本公司服务团队。(tech@proteometech.com)

Proteome Tech的蛋白质分析服务项目
Protein Characterization Services

Category Characterization method Use Quality attribute
Identity Peptide mapping
Total mass
Full length sequence
S-S bond localization
ID of post-translational modification
Primary structure
Protein fragments
Primary structure
Primary structure
Primary structure
Structure
Size
Structure
Structure
Structure
Purity SDS-PAGE
IEF
2D electrophoresis
HPLC (RP, SEC)
Protein fragments
Protein fragments
Protein fragments
Protein fragments
Size
Charge
Size, charge
Size, charge
Potency ELISA Potency Activity
Impurity Impurity profiling
Impurity ID
Impurities
Impurities
Impurities
Impurities
Glycan N-glycan profiling
Glycosylation site
Structure
Primary structure
Structure
Structure
Stability Analysis for stability test Test for stability Structure, activity
Other service Gel image analysis
MALDI-tof
LC-MS/MS
Column chromatography
De-glycosylation
ELISA
Identification
Identification, total mass
Identification
Protein purification
Glycan/protein analysis
Assay setup & validation

Western Blotting

Proteome Tech提供Western Blotting服务。

利用客户送来的蛋白质样品或者试料提供Western Blotting服务,采用客户送来的一级抗体(Primary antibody)和二级抗体(Secondary antibody)。
如果客户未提供一级抗体或者二级抗体时,由本公司按成本价提供。

Assay Development

本公司拥有研发基于ELISA的诊断方法的丰富经验。本公司研发团队曾研发过针对HBV、HCV、HIV、疟疾与过敏等各种疾病的诊断方法。
ELISA不仅可用作疾病的诊断方法,还可广泛用于蛋白质提炼、稳定性、生物医药品的QC等。按照客户需求和试料特性,可研发direct ELISA、indirect ELISA、sandwich ELISA等各种形态ELISA。

1.研发客户所需形式的assay方法
2.为客户提供明确的技术转让服务

* 所需费用和时间取决于课题难度。
* 欲知详情请致电本公司服务团队。 (tech@proteometech.com)

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