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Custom Protein Service

分析服务

分析服务
+82-2-323-8326 / tech@proteometech.com

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Protein Purification & Characterization

提炼蛋白质与特性描述

本公司对各类细菌与酵母细胞中发现的医药品重组蛋白质提炼工艺拥有丰富的经验。
不仅提供蛋白质提炼服务，还提供医药品研发过程中必需的蛋白质特性分析服务。

蛋白质提炼方法

Column chromatography
– Gel filtration chromatography
– Ion exchange chromatography
– Hydrophobicity chromatography
– Affinity chromatography
High-pressure Liquid Chromatography (HPLC)

蛋白质特性分析服务项目

SDS-PAGE
Isoelectric focusing
Western Blot analysis
ELISA
Intact mass determination (MALDI-TOF)
Peptide mapping
Post-translation modification analysis
Disulfide bond localization
Impurity identification
N-glycan Analysis

欲知详情请致电本公司服务团队。(tech@proteometech.com)

Proteome Tech的蛋白质分析服务项目
Protein Characterization Services

Category	Characterization method	Use	Quality attribute
Identity	Peptide mapping Total mass Full length sequence S-S bond localization ID of post-translational modification	Primary structure Protein fragments Primary structure Primary structure Primary structure	Structure Size Structure Structure Structure
Purity	SDS-PAGE IEF 2D electrophoresis HPLC (RP, SEC)	Protein fragments Protein fragments Protein fragments Protein fragments	Size Charge Size, charge Size, charge
Potency	ELISA	Potency	Activity
Impurity	Impurity profiling Impurity ID	Impurities Impurities	Impurities Impurities
Glycan	N-glycan profiling Glycosylation site	Structure Primary structure	Structure Structure
Stability	Analysis for stability test	Test for stability	Structure, activity
Other service	Gel image analysis MALDI-tof LC-MS/MS Column chromatography De-glycosylation ELISA	Identification Identification, total mass Identification Protein purification Glycan/protein analysis Assay setup & validation	–

Western Blotting

Proteome Tech提供Western Blotting服务。

利用客户送来的蛋白质样品或者试料提供Western Blotting服务，采用客户送来的一级抗体（Primary antibody）和二级抗体（Secondary antibody）。
如果客户未提供一级抗体或者二级抗体时，由本公司按成本价提供。

Assay Development

本公司拥有研发基于ELISA的诊断方法的丰富经验。本公司研发团队曾研发过针对HBV、HCV、HIV、疟疾与过敏等各种疾病的诊断方法。
ELISA不仅可用作疾病的诊断方法，还可广泛用于蛋白质提炼、稳定性、生物医药品的QC等。按照客户需求和试料特性，可研发direct ELISA、indirect ELISA、sandwich ELISA等各种形态ELISA。

1.研发客户所需形式的assay方法
2.为客户提供明确的技术转让服务

* 所需费用和时间取决于课题难度。
* 欲知详情请致电本公司服务团队。 (tech@proteometech.com)

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