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Date*

Institute/Dept*

Name*

Tel. No.*

E-mail*

Mobile No.*

Sample Information

Species*

Protein Name*

Conc. (mg/ml)*

Buffer (pH, Conc.)*

Composition*

Remark*

Protein Sample

cell lysate

protein

I.P Sample

Purified Sample

1DE Band(Coomassie Blue)

2DE Spot (Coomassie Blue)

1DE Band (silver)

2DE Spot (silver)

(L : LC-ms/ms, M : Malidi-TOF, Q : Q-TOF)

Protein Mass(M)

Protein I.D(L, Q)

Single Protein I.D(L, M)

1DE Band I.D(L, Q)

2DE Spot I.D(L, M)

Modification Analysis (L, Q)

I.P Protein I.D(L)

Purified Protein I.D(L, M)

Full sequencing(L, Q)

2DE

Large 2DE (18cm Gel and Dry Strip, More than 800 ug of protein Required)

Mini 2DE (7cm Gel and Dry Strip. About 150ug of Protein Required)

pH Range

pH3-10

Linear

Non-Linear

pH4-7

pH6-9

pH4.5-5.5

pH5.5-6.7

(We Recommend pH 4-7!!)

2D Gel Staining Method

Silver staining

CBB(Coomassie Blue G250) staining

(We Recommend CBB G250, If You Have More Than 800ug of Protein)

2D Gel Image analysis

Standard Analysis

Premium Analysis

Western Blotting

1DE W.B

2DE W.B

Primary Antibody Information

Secondary Antibody Information

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Sample Information

Protein Sample

(L : LC-ms/ms, M : Malidi-TOF, Q : Q-TOF)

Western Blotting

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