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Custom Protein Service

Contact Analytical Services

Analytical Services
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Protein purification & characterization

Protein purification ＆ characterization

We have accumulated extensive experience of purifying recombinant proteins from bacteria and yeast for protein drug. We can also provide protein characterization service for development of protein drug.

Purification Method

  • Column chromatography
    – Gel filtration chromatography
    – Ion exchange chromatography
    – Hydrophobicity chromatography
    – Affinity chromatography
  • High-pressure Liquid Chromatography (HPLC)

Characterization Analysis

  • SDS-PAGE
  • Isoelectric focusing
  • Western Blot analysis
  • ELISA
  • Intact mass determination (MALDI-TOF)
  • Peptide mapping
  • Post-translation modification analysis
  • Disulfide bond localization
  • Impurity identification
  • N-glycan Analysis

To request a quotation, please request a quotation form from customer services.(tech@proteometech.com)

Protein analysis service item of Proteometech
Protein characterization services

Category Characterization method Use Quality attribute
Identity Peptide mapping
Total mass
Full length sequence
S-S bond localization
ID of post-translational modification		 Primary structure
Protein fragments
Primary structure
Primary structure
Primary structure		 Structure
Size
Structure
Structure
Structure
Purity SDS-PAGE
IEF
2D electrophoresis
HPLC (RP, SEC)		 Protein fragments
Protein fragments
Protein fragments
Protein fragments		 Size
Charge
Size, charge
Size, charge
Potency ELISA Potency Activity
Impurity Impurity profiling
Impurity ID		 Impurities
Impurities		 Impurities
Impurities
Glycan N-glycan profiling
Glycosylation site		 Structure
Primary structure		 Structure
Structure
Stability Analysis for stability test Test for stability Structure, activity
Other service Gel image analysis
MALDI-TOF
LC-MS/MS
Column chromatography
De-glycosylation
ELISA		 Identification
Identification, total mass
Identification
Protein purification
Glycan/protein analysis
Assay setup & validation		 –

Western Blotting

Western Blotting Service

Proteometech performs your western blotting experiments on any type of biological material with antibodies and proteins that you supply or with ones that we have.

Assay Development

We provide extensive experience in the development of diagnostic assays. Our scientific team has comprehensive knowledge in developing sensitive assays for various virus or disease markers that include HBV, HCV, HIV, malaria, and allergy.

Enzyme-Linked ImmunoSorbent Assay (ELISA) is frequently used for protein purification, stability or lot release test. We can develop your ELISA assay using polyclonal or monoclonal antibodies. We will consult with you about your final use for the ELISA assay and discuss various formats that can be designed into your assay method. We provide contract ELISA development service that is adapted for direct ELISA, indirect ELISA or sandwich ELISA.

1. Specific assay(s) are developed to your specifications.
2. Technology transfers to the client upon project completion.
3. Cost and timeframe are dependent on the complexity of the project and level of our involvement.
4. To request a quotation, please request a quotation form from customer services. (tech@proteometech.com)

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